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. 2013 May 21;41(14):7073–7083. doi: 10.1093/nar/gkt423

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — Structural analysis of the 45S particles. (A) Surface representation of the cryo-EM map of the mature 50S subunit. (B–C) Surface representation of the 45S maps in the I-a (B) and II-a (C) states, superimposed with their missing components. (D–F) Surface representation of the cryo-EM maps of the mature 50S subunit (D), I-a (E) and II-a (F) states of the 45S particles, with their respective flexibly fitted atomic models superimposed. For simplification, only I-a and II-a states are shown. (G–I) Representative class averages of the cryo-EM raw images for the three conformational states (Mature, I-a and II-a), produced by a reference-free classification.