Table 4.
The kinetic parameters for the formation and decay of the pre-chemical Flp-DNA complexes and the conversion of synaptic complexes into recombinant products
| Wild type or mutant recombinase | Non-productive complex |
Wayward complex |
Pre-synaptic complex | Synaptic complex |
|||
|---|---|---|---|---|---|---|---|
| kf 104 M−1 s−1 | kd 10−2 s−1 | kf 10−2 s−1 | kd 10−2 s−1 | kf 104 M−1 s−1 | kf 10−2 s−1 | kr 10−3 s−1 | |
| Flpe | 18 ± 0.1 | 4 ± 0.3 | 1.7 ± 0.2 | 1.6 ± 0.1 | 6.0 ± 0.3 | 4.9 ± 0.4 | 1.7 ± 0.1 |
| Flpe (Y343F) | 3.1 ± 0.4 | 2.8 ± 0.3 | 2.2 ± 0.2 | 1.3 ± 0.1 | 1.5 ± 0.2 | – | – |
The rate constants for the individual steps, derived from the dwell times of DNA molecules bound by Flpe or Flpe(Y343F) in the respective precursor states, are indicated.
The rate constants for the formation and decay of a given complex are denoted as kf and kd, respectively.
The rate constant of recombination kr describes the formation of recombinant product(s) from the synaptic complex.
The values for kf and kd are for the excision reaction from direct FRT sites diagrammed in Figure 6.
The value for kr is for the inversion reaction from inverted FRT sites, based on a double exponential model for the decay of wayward complexes and formation of the recombinant product from the synapsed state (see text for details).