Figure 5.

Centrin-2 augments physical interaction between XPC and XPA. (A) Biotinylated XPC/RAD23B immobilized on paramagnetic beads was used to pull down purified TFIIH and/or XPA in the presence or absence of WT centrin-2. (B) XPA was pulled down with paramagnetic beads pre-bound to XPC/RAD23B with or without centrin-2 (WT, N or C). The amounts of XPA pulled down were quantified and shown as relative values (average values were calculated from two experiments). (C) Centrin-2, WT, C domain or M4 mutant, was pulled down with FLAG-XPA immobilized on anti-DDDDK-tag Magnetic beads. In each experiment, co-precipitated proteins were subjected to immunoblotting with the indicated antibodies.