Figure 6.

Centrin-2 is required for efficient UV-induced recruitment of XPA to chromatin sites where XPC is bound. Two stably transformed cell lines of XP4PASV expressing FLAG-XPC (WT or CBM) were treated with UVC (10 J/m²). (A) Cross-linked chromatin fragments were prepared at various time points and subjected to ChIP assays with the anti-FLAG M2 magnetic beads. Co-precipitated proteins were detected by immunoblot analyses using the indicated antibodies. (B) The amounts of XPB and XPA in the ChIP fractions were quantified and plotted as relative values (the values from non-irradiated samples were set as 1). Average values and standard errors were calculated from three independent experiments.