Skip to main content
. 2013 May 28;41(14):e139. doi: 10.1093/nar/gkt442

Figure 2.

Figure 2.

Effects of insert size on recombination and minicircle formation. (A) Substrate BV constructs carrying stuffer DNA of different sizes. (B) d2egfp copy numbers. (C) Minicircle copy numbers. HEK293 cells were co-transduced with BacCre (or BacFLPo, MOI 100) and the substrate BV (BacALF, BacALF-5k, BacALF-10k or BacALF-15k) at MOI 200. At 1 dpt, the absolute copy numbers of d2egfp and minicircles were analyzed by qPCR using d2egfp- and minicircle-specific primers, respectively. The insert sizes were actually 2.2, 5.3, 10.4 and 15.4 kb, respectively, but are shown as 2, 5, 10 and 15 kb for simpler presentation.