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. 2013 May 28;41(14):e139. doi: 10.1093/nar/gkt442

Figure 5.

Figure 5.

Prolonged growth factor expression and acceleration of osteogenesis. (A) Schematic illustration of BacL-CB/W-CEO and BacL-CV/W-CEO. (B) Duration of BMP2 and VEGF expression as measured by ELISA. hASCs were mock-transduced, singly transduced with the growth factor-expressing hybrid BV (MOI 200) or co-transduced with BacCre (MOI 100) and the hybrid BV (MOI 200). (C) Transcription profiles of osteogenesis markers. The mRNA levels of four osteogenesis marker genes were measured by qRT-PCR at 7 and 14 dpt and normalized against those at 0 dpt. (D) Calcium deposition as stained by Alizarin red. Magnification, 100×.