Figure 2.

Heritable chromosomal deletions in zebrafish in the region of miRNA genes and miRNA gene clusters mediated by two pairs of TALENs. (A) The structure of the four loci of two zebrafish miRNA genes dre-mir-126a and dre-mir-126b and two miRNA gene clusters located on Chr. 1 and Chr. 9, with the approximate locations and distances of TALEN target sites and primers for PCR detection. (B) PCR results of the genomic DNA from injected (two TALENs) and un-injected (WT) embryos. For the loci of dre-mir-126a and dre-mir-126b, the PCR products of both wild-type sequences (white arrowheads) and sequences containing deletions (black arrowheads) can be seen. For the loci of the two miRNA gene clusters (the two lower panels), only the sequences carrying deletions (black arrowheads) can be amplified with the PCR program used in this experiment. Only one combination of TALEN pairs is shown here for each locus. Results of other combinations of TALENs for the same locus can be found in Supplementary Figures S2 and S4.