Figure 3.

Interplay between FA proteins and ATRIP. (A) Yeast two-hybrid interaction assay between ATRIP and various full-length FA or FA-related proteins. Selected yeast transformants were spotted onto SD/-Ura/-His/-Trp/-Leu/+X-gal plates at 30° for 96 h. ATRIP–ATRIP interaction served as positive control. (B) Co-immunoprecipitation assay between overexpressed human FANCL-GFP or GFP-FAAP100 and ATRIP-Flag in 293T cells. F or G, Flag or GFP vector without insert. (C) RPA-ssDNA pull-down assay from extracts of FA-A (GM6914) and complemented FA-A cells (+A). ssDNA-attached magnetic beads preincubated with or without recombinant RPA were mixed with cell extracts. After washing, the bound material was analyzed by western blotting.