Figure 2.

¹H-¹⁵N TROSY spectra of the RRM123 construct at 800 MHz. Overlayed spectra of the unbound RRM123 (blue) with RRM123 complexes with EDEN11 (red) and EDEN-4U (green) at an RNA:protein ratio of 3:1. The region shown highlighted in (a) containing cross-peaks of some Gly and Cys residues is expanded in (b). Key residues from all three RRMs that show significant CSP effects on RNA binding are labelled. The proposed binding stoichiometry is shown schematically.