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. 2013 May 30;41(14):6942–6951. doi: 10.1093/nar/gkt475

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Endogenous p21 delays the recruitment of pols in the Y family to DNA replication-associated foci. (A) HCT116 p21+/+ and p21−/− were UV irradiated (30 J/m²) and lysed at the indicated time points. Western blots were performed using p21, PCNA and actin antibodies. (B) HCT116 p21+/+ and p21−/− cells transfected with GFP-pol η were fixed at different time points after UV irradiation (50 J/m²), and the sub-nuclear distribution of pol η was determined in three independent experiments. Similar experiments were performed for pol ι (C), pol κ (D) and Rev1 (E). In all, 200 cells/sample were counted. Significance of the differences between p21+/+ and p21−/− cells in each condition. **P < 0.01, *: P < 0.05, no asterisk = NS—not significant, P > 0.05.