Skip to main content
. Author manuscript; available in PMC: 2013 Aug 8.
Published in final edited form as: Anticancer Res. 2012 Aug;32(8):3103–3113.

Figure 7.

Figure 7

β-Elemene triggered caspase activation and cleavage in A2780 and A2780/CP cells. A and B: Caspase-3, -8 and -9 enzymatic activities in A2780 and A2780/CP cells were measured using a CasPASETM apoptosis assay kit. Cells were treated with β-elemene (ELE) at 30, 50, and 70 μg/ml for 48 h, harvested, and washed twice with ice-cold PBS. Cytosolic extracts were prepared and incubated with 5 μl of 1 mM caspase substrate at 37°C for 2 h. Absorbance of the cleaved substrate was measured at 405 nm. Untreated cell lysate was used as a control. Relative caspase activity was determined by calculating the fold increase of caspase activity in β-elemene-treated cells vs. untreated cells. *p<0.05 and #p<0.01 vs. the untreated groups. C: Caspase-9 cleavage in A2780 and A2780/CP cells is seen on western blots.