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. Author manuscript; available in PMC: 2014 Jul 24.
Published in final edited form as: Neuron. 2013 Jul 3;79(2):322–334. doi: 10.1016/j.neuron.2013.05.012

Figure 4. Diffuse ACs receive excitatory input and depolarize during the ON phase of stage III waves.

Figure 4

(A) Circuit diagram of the retina in which neurons recorded for this figure are highlighted; labeling as in Figure 1. (B) Orthogonal projections of a 2-photon image stack of a diffuse AC (green) and OFF RGC (magenta) filled during a recording. (C) Simultaneous current- (I = 0 pA, VRest ~ −46 mV) and voltage-clamp clamp (EPSCs, VM ~ −60 mV) recording from a diffuse AC and ON RGC, respectively. (D) The mean maximal voltage change during waves for each diffuse AC is indicated by open circles. A solid line shows the mean of all diffuse ACs (n = 18) analyzed. (E) Excerpts of the traces in (C) on an expanded timescale. (F) Crosscorrelation (mean ± SEM, n = 9) of the membrane potential (v) of diffuse ACs with excitatory conductances in ON RGCs (or inhibitory conductances in OFF RGCs) (g). (G) Simultaneous recording of EPSCs in a diffuse AC and an ON RGC. (H) Crosscorrelation (mean ± SEM, n = 5) of excitatory conductances in diffuse ACs (g) with excitatory conductances in ON RGCs (or inhibitory conductances in OFF RGCs) (g).