Fig. 7.

β1 integrin-dependent regulation of global gene expression in RIP-Cre/β1KO sorted β-cells. (A,B) Heat maps of selected genes involved in cell adhesion (A) or the cell cycle (B) that are downregulated or upregulated in RIP-Cre/β1KO sorted β-cells. (C) Altered expression levels of cell cycle genes plotted onto the cell cycle pathway map using GenMAPP software. (D) Changes in expression of the key regulators of cell cycle progression cyclin D1 and Cdkn1a (p21) validated by qPCR. Values are expressed as fold expression ± s.d. relative to WT (WT value=1), and normalized to Gapdh. ^**P<0.01. (E) Western blot analysis of isolated pancreatic islets probed for phospho-ERK1/2, phospho-PKB, PTEN and α-tubulin. (F) Densitometric quantification of immunoblot bands from E. Values are representative of three independent experiments and are expressed as fold expression ± s.d. relative to WT and normalized to β-actin. ^**P<0.01.