Fig. 3.

bbof1 morphants respond to flow but cannot maintain a precise orientation. (A) The experimental design for treating ventral explants with an external flow is diagrammed. (B,C) Average ciliary flow produced by four explants from control (B) or bbof1 morphants (C) is shown, when measured pre-flow treatment (Pre), 0.5 hours post-flow treatment (Post) or after 5 hours of recovery (Rev). Error bars indicate ±s.d. (D-I) Cilia orientation in MCCs was measured and expressed in polar plots as described in the legend to Fig. 2, in explants before flow treatment (D,G), just after flow treatment (E,H) or after 5 hours of recovery (F,I). Arrow colors represent MCCs taken from the same explant. Each plot is oriented with anterior (cement gland) towards the left and posterior (proctodeum) towards the right. Value in each panel equals average arrow radius±s.d. for a given condition.