Fig. 5.

Ras/MAPK signaling is inhibited in the ndk-1(-) mutant background. Analysis of total and diphosphorylated MAPK levels of wild-type and ndk-1(ok314) worms by western blotting. Black arrows indicate the germline-specific (55 kDa) isoform of MAPK; red arrows show the somatic (45 kDa) isoform of MAPK. As a control for loading, the blot was reprobed using anti-tubulin antibody. The mutant germline completely lacks MAPK. The level of total MAPK in the soma is comparable in mutant and wild-type worms. However, the level of activated MAPK is severely reduced in ndk-1(ok314) mutants.