Figure 4.

Copy number limits of 30 cell-cycle regulators in S. pombe. The green bar indicates the plasmid copy number obtained in the gTOW experiment with each vector. The blue bar indicates the maximum plasmid copy number for each target gene obtained in the gTOW experiment with three different vectors. The orange bar indicates the maximum plasmid copy number for each target gene with frameshift mutation. The original data are shown in Supplementary Table S6. Because the cell has an endogenous copy of the target gene, the copy number limit for the target gene is the plasmid copy number plus 1.