Table 3.

P-values of t-tests for immunostaining score between 12 h of 10% NBF fixation or 10% NBF for 12 h followed by transfer to 70% ethanol at experimental time points for DU145 and SKOV3 after cells

	12 (h) in 10% NBF + EtOH (h)	12 + 0	12 + 3	12 + 6	12 + 24	12 + 96	12 + 168
PCNA
DU145	12 + 0	–	1.0	0.58	1.0	0.97	0.80
SKOV3	12 + 0	–	1.0	1.0	1.0	1.0	1.0
Cytokeratins AE1/AE3
DU145	12 + 0	–	0.20	0.18	0.72	0.07	0.25
SKOV3	12 + 0	–	0.12	0.52	0.77	0.86	0.37
EGFr cytoplasmic
DU145	12 + 0	–	0.96	0.99	0.98	0.99	0.81
SKOV3	12 + 0	–	1.0	0.99	1.0	0.98	1.0
EGFr membrane
DU145	12 + 0	–	1.0	1.0	1.0	1.0	1.0
SKOV3	12 + 0	–	1.0	1.0	1.0	1.0	1.0
Ki67-MIB-1
DU145	12 + 0	–	0.36	1.0	0.99	0.98	0.82
SKOV3	12 + 0	–	1.0	1.0	1.0	1.0	1.0

Data are from three replicate experiments.