Table 2.

Geometric analysis of the effect of sensory deprivation on somatic, apical dendritic, and basilar dendritic parameters in pyramidal neurons

Domain	Metric	Mean sensory deprived n = 39	SD sensory deprived	Mean control n = 35	SD control	t value (indep, student)	p value
Somatic	Perimeter (µm)*	66.24	12.04	58.28	1.60	3.136	0.001
Somatic	Area (µm2)*	239.88	66.16	193.90	8.39	3.351	0.001
Somatic	Feret max (µm)a	22.55	4.03	19.99	0.54	2.999	0.002
Somatic	Feret min (µm)b	16.18	3.09	14.40	0.45	2.648	0.005
Somatic	Aspect ratioc	1.4200	0.2699	1.4112	0.0383	0.151	0.440
Somatic	Compactnessd	0.7773	0.0751	0.7904	0.0135	−0.728	0.234
Somatic	Convexitye	0.9379	0.0541	0.9551	0.0067	−1.554	0.062
Somatic	Form factorf	0.6976	0.1254	0.7286	0.0195	−1.102	0.137
Somatic	Roundnessg	0.6098	0.1170	0.6310	0.0212	−0.753	0.227
Somatic	Solidityh	0.9030	0.0628	0.9191	0.0095	−1.154	0.126
Apical den.	Quantity	1	0	1	0
Apical den.	Nodes	3.36	2.47	6.09	0.75	−3.320	0.001
Apical den.	Ends*	4.26	2.54	7.54	0.77	−3.902	0.000**
Apical den.	Length (µm)*	313.97	151.02	529.87	47.66	−4.165	0.000**
Apical den.	Surface area (µm2)*	1553.80	713.53	2492.17	193.88	−4.272	0.000**
Apical den.	Volume (µm3)*	671.48	299.03	1103.80	87.67	−4.448	0.000**
Basilar den.	Qty	6.46	3.75	5.14	0.45	1.730	0.044
Basilar den.	Nodes*	7.79	9.13	3.11	0.34	2.968	0.002
Basilar den.	Ends*	14.33	11.96	8.11	0.53	2.983	0.002
Basilar den.	Length (µm)*	640.78	383.28	395.06	38.00	3.314	0.001
Basilar den.	Mean leni	108.06	78.84	93.39	9.75	0.905	0.184
Basilar den.	Surface (µm2)	2350.31	1568.56	1505.21	156.63	2.781	0.003
Basilar den.	Mean suri	402.34	336.90	339.31	38.16	0.934	0.177
Basilar den.	Volume (µm3)	760.09	561.90	493.98	55.60	2.449	0.008
Basilar den.	Mean voli	131.43	120.80	110.75	13.61	0.856	0.197
Total (apical + basilar)	TD-Qtyj	7.46	3.75	6.14	0.45	1.73	0.044
Total (apical + basilar)	TD-nodesj	11.15	10.28	9.20	0.96	0.996	0.161
Total (Apical + Basilar)	TD-endsj	18.59	13.05	15.66	1.09	1.203	0.117
Total (apical + basilar)	TD-length (µm)j	954.75	482.02	924.93	74.23	0.277	0.391
Total (apical + basilar)	TD-surface area (µm2)j	3904.12	2013.13	3997.37	306.34	−0.208	0.418
Total (apical + basilar)	TD-volumej	1431.56	744.21	1597.78	123.80	−0.966	0.169

Bold indicates statistical significance at p<0.05

^*Statistical significance at α level preset = 0.05

^**p value less than 0.0001

^aFeret maximum: longest diameter of the soma

^bFeret minimum: longest diameter perpendicular to feret maximum

^cAspect ratio: (feret max)/(feret min); as aspect ratio approaches 1, it is indicative that the soma is closer to a symmetric shape (e.g., circle or square)

^dCompactness: [√(4/π) × area]/(feret max); numerical values of somatic compactness closer to 1 represent a more perfect spherical soma

^eConvexity: (convex contour)/(perimeter); this parameter is indicative of the somatic surface areal profile; higher somatic convexity yields to more indentations, which translates to higher estimated surface area to cellular volume ratio

^fForm factor: (4π × area)/(perimeter²); this value directly reflects the complexity of somatic perimeter; a higher numerical value represents a more complex somatic perimeter

^gRoundness: (4 × area)/(π × feret max²), similar to compactness but is another way to differentiate objects with small compactness values for the closeness to a perfect sphere

^hSolidity: the ratio of somata area as a whole over convex area, where values closer to 1 represent more solid (i.e., smooth, uniform) somata

ⁱDendritic mean length, mean surface area, and mean volume: are all derived from total dendritic length, total surface area, and total dendritic volume divided by the number of primary dendritic trunks, respectively

^jTotal dendritic variables (Qty, Nodes, Ends, Length, Surface Area, Volume): These are all derived from adding apical and basilar dendritic components together, e.g., total dendritic length = apical dendritic length + basilar dendritic length