Table 1. List of solved crystal structures for small Ras superfamily GTPase/effector domain complexes.
| GTPase superfamily subdivisions |
Effector binding proteins |
Crystal structures |
GTPase effector protein/effector domain crystal structures |
|---|---|---|---|
| Arf subfamily | |||
|
Arf1 |
ARHGAP21 |
ARHGAP21ArfBD (PH domain and helical region) in complex with Arf1 [17347647] |
Arf1/ARHGAP21 Arf-binding domain (ArfBD) |
| |
GGA |
GGAGAT domain (N-terminus) in complex with Arf1 [12679809] |
Arf1/GGA GAT domain |
|
Arf6 |
Cholera toxin (CT) |
CTA1 subunit in complex with Arf6 [16099990] |
- |
| |
JIP4 |
JIP4LZII in complex with Arf6 [19644450] |
Arf6/JIP4 leucine zipper II (LZII) |
| |
MKLP1 |
MKLP1C in complex with Arf6 [22522702] |
- |
|
Arl1 |
Arfaptin-2 |
Arfaptin-2BAR Domain in complex with Arl1 [22679020] |
Arl1/Arfaptin-2 BAR domain |
| |
Golgin-245 |
Golgin-245GRIP Domain in complex with Arl1 [14718928] |
Arl1/Golgin-245 GRIP domain |
|
Arl2 |
BART |
BART in complex with Arl2 [19368893] |
- |
| |
PDEδ |
PDEδC in complex with Arl2 [11980706] |
- |
| |
UNC119a |
UNC119a in complex with Arl2 [22960633] |
- |
|
Arl3 |
UNC119a |
UNC119a in complex with Arl3 [22960633] |
- |
|
Rho subfamily |
|
|
|
|
Cdc42 |
ACK |
ACKCRIB in complex with Cdc42 [10360579] |
Cdc42/ACK CRIB domain |
| |
PAK |
PAKCRIB in complex with Cdc42 [10802735] |
Cdc42/PAK CRIB domain |
| |
Par6 |
Par6CRIB-PDZ in complex with Cdc42 [12606577] |
Cdc42/Par6 CRIB-PDZ domains |
| |
WASP |
WASPCRIB in complex with Cdc42 [10360578] |
Cdc42/WASP CRIB domain |
|
Rac1 |
Phospholipase C-β2 |
Phospholipase C-β2ΔC (PH-EF-TIM-C2 domain-containing fragment) in complex with Rac1 [17115053] |
Rac1/Phospholipase C-β2 PH domain |
| |
Plexin-B1 |
Plexin-B1ΔN in complex with Rac1 [ 21912513] |
- |
|
Rac2 |
Phospholipase C-γ2 |
Phospholipase C-γ2spPH in complex with Rac2 [19394299] |
Rac2/Phospholipase C-γ2 split PH domain |
|
RhoA |
PKN |
PKNN (ACC finger domain) in complex with RhoA [10619026] |
RhoA/PKN atypical coiled-coil (ACC) domain |
| |
ROCK1 |
ROCK1RhoBD (coiled-coil) in complex with RhoA [14660612] |
- |
|
RhoC |
Dia1 |
Dia1N (GBD) in complex with RhoC [15864301; 16292343; 16472745] |
RhoC/Dia1 GTPase-binding domain (GBD) |
|
Rnd1 |
Plexin A2 |
Plexin A2RhoBD (ubiquitin-like fold) in complex with Rnd1 [21610070] |
Rnd1/Plexin A2 Rho-binding domain (RhoBD) or RBD-like |
| |
Plexin B1 |
Plexin B1RhoBD in complex with Rnd1 [21610070] |
Rnd1/Plexin B1 Rho-binding domain (RhoBD) or RBD-like |
|
Rnd3 |
ROCK1 |
ROCK1N in complex with Rnd3 [18946488] |
- |
|
Ras subfamily |
|
|
|
|
H-Ras |
PI3K |
PI3KγRBD in complex with H-Ras [11136978] |
H-Ras/PI3Kγ Ras-binding domain (RBD) |
| |
RalGDS |
RalGDSRBD in complex with H-Ras [9628477; 10371160] |
H-Ras/RalGDS Ras-binding domain (RBD) |
|
RalA |
C3bot1 |
C3bot1 in complex with RalA [15809419] |
- |
| |
Exo84 |
Exo84RalBD (PH-FOLD) in complex with RalA [15920473] |
RalA/Exo84 PH domain |
| |
Sec5 |
Sec5RalBD (immunoglobulin-like β-sandwich) in complex with RalA [12839989] |
RalA/Sec5 immunoglobulin-like β-sandwich |
|
Rap1A |
c-Raf1 |
c-Raf1RBD (ubiquitin-like fold) in complex with Rap1A [7791872] |
Rap1A/c-Raf1 Ras-binding domain (RBD) |
| |
Krit1 |
Krit1FERM domain in complex with Rap1 [22577140] |
Rap1/Krit1 FERM F1 and F2 lobes |
|
Rab subfamily |
|
|
|
|
Rab5A |
EEA1 |
EEA1C2H2 ZF in complex with Rab5A [20534488] |
Rab5A/EEA1 C2H2 zinc finger (ZF) |
|
Rab6 |
Rab6IP1 |
Rab6IP1α-helical RUN-PLAT domains in complex with Rab6 [19141279] |
Rab6/Rab6IP1 RUN domain |
|
Rab8A |
OCRL1 |
OCRL1ASH domain in complex with Rab8A [21378754] |
Rab8A/OCRL1 ASH domain |
| Rab22A | Rabenosyn5 | Rabenosyn5C2H2 ZF in complex with Rab22A [20534488] | Rab22A/C2H2 zinc finger (ZF) |
ACC, antiparallel coiled-coil; ArfBD, Arf-binding domain; ASH, ASPM/SPD-2/Hydin; BAR, Bin1/Amphiphysin/Rvs167; C, C-terminus; CRIB, Cdc42/Rac interactive binding; FERM, 4.1 protein/Ezrin/Radixin/Moesin; GAT, GGA and Tom1; GBD, GTPase-binding domain; GRIP, Golgin-97/RanBP2alpha/Imh1p/p230/golgin-245; LF, leucine finger; LZ, leucine zipper; N, N-terminus; PDZ, PSD-95 and ZO-1; PH, pleckstrin homology; RBD, Ras-binding domain; RhoBD, Rho-binding domain; RUN, RPIP8/UNC-14/NESCA; spPH, split PH; ZF, zinc finger. Numbers in brackets denote Pubmed IDs (PMIDs).