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. Author manuscript; available in PMC: 2013 Aug 8.
Published in final edited form as: J Biol Chem. 2007 May 11;282(31):22804–22815. doi: 10.1074/jbc.M700961200

FIGURE 4. The monoubiquitination-mimicking p53 fusion protein can form tetramers in an oligomerization assay.

FIGURE 4

A equal amounts of total protein were incubated with 0, 0.01 %, and 0.1% gluteraldehyde on ice for 30 min and resolved on a 7% SDS-PAGEgel followed by Western blot using anti-p53 (DO-1) antibody. B, the in vitro oligomerization assay was repeated using purified GST-p53-UB protein incubated with increasing amounts of gluteraldehyde and resolved on a 7% SDS-PAGE followed by Western blot using DO-1.