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A, Western blot analysis of p53 inputs taken from the ChIP experiment in Fig. 9B. 8% SDS-PAGE was performed with pCIN4 (lane 1), p53 (lane 2), p53 and Mdm2 (lane 3), p53 and Mdm2 in the presence of the proteasome inhibitor MG132 (lane 4), or p53 and Mdm2(C464A) (lane 5) immunoblotted with DO-1. B, ChIP assay using anti-p53 (FL)(lanes 3, 6, 9, 12, and 15) or IgG (lanes 2, 5, 8, 11, and 14) compared with input DNA (lanes 1, 4, 7, 10, and 13).
