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β2*-nAChR occupancy by nicotine after IV nicotine administration on two SPECT scan days: 1. before immunization (left – closed circles) and 2. after immunization (open circles) connected by a line to represent each subject. There was a significant average 12.5% decrease in nicotine’s binding to the receptors after the immunization. Difference in binding was calculated as [Ro after / Ro before] * 100= % difference.
