A composite (A) and the expanded bird’s-eye view (B) images show DTI revealed cell alignment in a WT mouse eye. Cell alignments were color coded to differentiate cells aligned more parallel to (≤ 45°, blue) or perpendicular to (> 45°, red) the retina or lens surface. A picrocirous red (C, left) and an H&E (C, right) stained retina sections of a WT mouse show that photoreceptor nuclei in ONL and inner and outer segments in IS/OS are aligned nearly perpendicular to the retinal surface. A picrocirous red stained lens section of the WT mouse shows fiber cells in the lens cortex are aligned parallel to the lens surface (D). RPE, retinal pigment epithelium.