Table 2.
Summary of down regulated genes encoding cell wall functions in goa1Δ
| Gene | Fold | Function |
|---|---|---|
| Cell wall biogenesis/integrity (n=40) | ||
| orf19.1160, orf19.5412, | 0.28-0.29 | fungal-type cell wall organization |
| orf19.719 | 0.37 | |
| orf19.4984 | 0.47 | chitin catabolic process |
| orf19.1196 | 0.44 | role in MAPKKK cascade involved in cell wall biogenesis |
| Orf19.4581 | 0.48 | glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I |
| ADA2 | 0.24 | transcriptional coactivator; cell wall integrity role |
| CHK1 | 0.21 | histidine kinase, regulates cell wall biosynthesis |
| CHT1,3 | 0.39, 0.27 | chitinase |
| CRH11 | 0.33 | GPI-anchored cell wall transglycosylase |
| ECM1,4,7,15,17,21,331 | 0.16-0.48 | cell wall organization or biogenesis |
| ENG1 | 0.43 | endo-1,3-beta-glucanase |
| FKS1 | 0.49 | beta-1,3-glucan synthase subunit |
| MNN4, orf19.5557 | 0.27-0.49 | mannosyltransferase |
| MNT4 | 0.45 | mannosyltransferase |
| PHR1,3 | 0.46, 0.20 | beta-1,3-glucanosyltransferase |
| PIR1 | 0.34 | 1,3-beta-glucan-linked structural cell wall protein |
| IRS4 | 0.28 | roles in cell wall integrity |
| KIC1 | 0.47 | Ste20p kinases; in RAM cell wall integrity signaling network |
| PGA10,31,52 | 0.25-0.47 | GPI anchored cell surface protein |
| SLK19 | 0.39 | alkaline-induced membrane protein; affects cell aggregation |
| SMI1 | 0.48 | cell wall biosynthesis protein |
| SUR7 | 0.38 | required for normal cell wall, plasma membrane |
| XOG1 | 0.18 | exo-1,3-beta-glucanase |
| BGL22 | 0.21 | glucanase; induced during cell wall regeneration |
| BMT3 | 0.08 | beta-mannosyltransferase |
| EXG2 | 0.44 | GPI-anchored cell wall protein |
| Orf19.5070 | 0.42 | cell-wall mannoproteins |
| VPS28 | 0.49 | proteolytic activation of Rim101p, role in echinocandin sensitivity |
| Cell membrane associated genes (n=6) | ||
| PLB5 | 0.42 | GPI-linked phospholipase B |
| YPT31 | 0.50 | required for resistance to toxic ergostero analog |
| ERG27 | 0.42 | 3-Keto sterol reductase of ergosterol biosynthesis |
| Orf19.3226 | 0.28 | intracellular sterol transport |
| orf19.4096 | 0.06 | acylglycerophosphocholine O-acyltransferase |
| orf19.7547 | 0.27 | phosphatidylinositol-3-phosphate binding |
| Adhesin/antigenic genes (n=26) | ||
| ALS1, 2,3,4,5,6,7 | 0.08-0.49 | ALS family protein, cell-surface glycoprotein, adhesion |
| IFF4,5,8,11 | 0.11-0.43 | GPI-anchored protein , adhesion-like protein |
| HSP70 | 0.15 | antigenic, role in entry into host cells |
| HYR3 | 0.45 | adhesion-like protein |
| orf19.251 | 0.30 | binds human immunoglobulin E |
| AHP1 | 0.42 | alkyl hydroperoxide reductase; immunogenic in mouse |
| CSH1 | 0.38 | aldo-keto reductase family member, role in fibronectin adhesion |
| CYC3 | 0.50 | cytochrome c heme lyase, antigenic cell-wall protein |
| DDR48 | 0.05 | immunogenic stress-associated protein |
| MET15, 6 | 0.04, 0.23 | antigenic during murine or human systemic infection |
| PGA62 | 0.44 | adhesin-like cell wall protein |
| RBR3 | 0.37 | cell wall adhesin-like protein |
| PST2 | 0.07 | NADH:quinone oxidoreductase, immunogenic in mice |
| SAP10, 9 | 0.22, 0.24 | secreted aspartyl protease; roles in adhesion |
| XYL2 | 0.16 | D-xylulose reductase; immunogenic in mice |
| Yeast-hyphal morphological transition genes (n= 14) | ||
| CEK1 | 0.44 | ERK-family protein kinase, yeast-hyphal switching |
| CHO1 | 0.33 | phosphatidylserine synthase, required for filamentous growth |
| CSP37 | 0.25 | hyphal cell wall protein |
| DFG5 | 0.28 | N-linked mannoprotein, role in hyphal growth |
| GPH1 | 0.28 | glycogen phosphorylase, localizes to cell surface of hyphae |
| MID1 | 0.38 | high affinity calcium uptake system, role in thigmotropism |
| PHO85,111 | 0.50,0.50 | acid phosphatase, negatively regulated by Rim101p |
| RBT5 | 0.09 | GPI-anchored cell wall protein |
| RHD3 | 0.32 | GPI-anchored cell wall protein; yeast-associated protein |
| RVS167 | 0.40 | involved in endocytosis; hyphal growth required |
| SAM2 | 0.50 | S-adenosylmethionine synthetase, localizes to surface of hyphal cells |
| YDC1 | 0.21 | Mob2p-dependent hyphal regulatio |
| SNZ1 | 0.12 | induced on yeast to hyphal switch |