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. 2013 Aug 8;33(4):e00056. doi: 10.1042/BSR20130045

Figure 1. SDS–PAGE and native PAGE of wt and mutant HMBS.

Figure 1

(A) SDS–PAGE showing recombinant HMBS as purified: Wt (lane 1), R116W (lane 2), K132N (lane 3), R167W (lane 4), R173W (lane 5) and V215E (lane 6). Low molecular mass SDS–PAGE standards (lane 7). (B) Native PAGE of recombinant HMBS as purified: Wt (lane 1), R116W (lane 2), K132N (lane 3), R167W (lane 4), R173W (lane 5) and V215E (lane 6). See the main text for the interpretation of protein bands.