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. 2013 Aug 8;33(4):e00056. doi: 10.1042/BSR20130045

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(A) Far-UV CD of wt (○), R116W (▲), K132N (∆), R167W (□), R173W (●) and V215E (■). [θ], mean residue ellipticity. (B) The CD-monitored thermal denaturation, presented as mdeg at 222 nm versus temperature. (C) The CD-monitored thermal denaturation, normalized to fraction of unfolded protein, and curve fitting to a two-state transition model for wt (1), R116W (2), K132N (3), R167W (4), R173W (5) and V215E (6). (D) Thermal denaturation monitored by DSF for wt (○), R116W (▲), K132N (∆), R167W (□), R173W (●) and V215E (■). A representative plot of each of four parallels is shown in the figure. The T_m-values obtained from (C) and (D) are summarized in Table 4.