Table 4. The T_m for wt HMBS and mutants as measured by CD and DSF.

	Tm-values (°C)
Mutation	CD*	DSF†	DSF, Tm1‡	DSF, Tm2‡
Wt	74.0±0.1	71.1±0.6	54.4±0.8§	72.8±0.3
R116W	53.1±0.4	42.6±2.1	42.1±1.7	−
K132N	74.1±0.3	69.4±0.3	53.3±0.5§	71.0±0.2
R167W	74.3±0.0	67.8±0.2	48.2±0.9§	70.6±0.1
R173W	60.3±0.2	44.5±0.6	45.6±0.4	62.8±0.1
V215E	74.5±0.3	54.7±2.8	43.2±0.5	70.3±0.4

*Determined at fraction of unfolded=0.5 by curve fitting of the thermal transitions [35,36]. See Figure 3(B).

^†Determined at fraction of unfolded=0.5 in the thermal transitions. See Figure 3(C).

^‡T_m1 and T_m2 were calculated from the first derivative using the transitions observed in the temperature scans. See Figure 3(D).

^§Minor transitions.