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Efficiency and specificity of Taqman miRNA assays. (A) PCR efficiencies for each assay in either single plex or multiplexed reactions, with and without pre-amplification. (B) Specificity of the miRNA assays. Cross-reactivity between the assays was calculated based on the Cq difference between assay-specific (set to 100%) and non-specific synthetic miRNA targets. Values for the two cases of detectable cross-reactivity are shown on the graph.
