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DPEP1 mRNA expression is associated with CRC localisation and stage. The mRNA of validation cohort 1 was isolated, reverse transcribed and the relative expression levels were analysed by real-time PCR. Relative expression levels of DPEP1 and SDCBP2 were normalised to GAPDH. Data represent the mean mRNA expression plotted as the fold-change compared with control tissue. (A and C) Tumour localisation and (B and D) tumour stage. Tu, tumour tissue; No, normal non-cancerous tissue.
