Figure 6.

MICA, MICB and ULBP2 could reverse the effects of miR-302c- and miR-520c-mediated resistance of malignant cells to NK cells upon 1,25(OH)2D3 treatment. (A) MDA-MB-231 cells transfected with indicated miRNAs and vectors for 24 h were treated with 0.1 μM 1,25(OH)₂D₃ for another 24 h and then co-incubated with NK92 cells at an E:T ratio of 16 : 1 for 4 h. The killing effects of NK92 cells against MDA-MB-231 cells evaluated using the CytoTox 96 Non-Radioactive Cytotoxicity Assay. (B) The supernatant was collected and subjected to the measurement of IFN-γ. The results are expressed as the means±s.d.; n=3, *P<0.01 compared with the control.