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. 2013 Apr 29;20(4):355–374. doi: 10.1093/dnares/dst015

Figure 2.

Figure 2.

Allelic variation detected among a representative set of desi and kabuli genotypes using normal unlabelled and fluorescent dye-labelled TFGMS and TFFDMS markers in gel-based assay (A) and automated fragment analyzer (B), respectively. A maximum of 8 and 10 polymorphic alleles were amplified by markers among 96 chickpea genotypes using the gel-based assay (A) and automated fragment analyzer (B), respectively. The fragment sizes (bp) for all the amplified polymorphic alleles are indicated. The sequencing of cloned amplicons from different TFGMS and TFFDMS markers showing fragment length polymorphism validated the presence of expected microsatellite repeat motifs and further corresponded with their variations and step-wise multiples of the number of repeat units.