FIGURE 3.

Microgliosis but not astrogliosis was attenuated in the brains of male APP/PS1 mice supplied with 0.625% pomegranate in their normal drinking water for 3 mo of treatment compared with 12- and 15-mo-old male control APP/PS1 mice provided normal drinking water. (A) Coronal sections were immunostained by using anti-CD68 and anti-GFAP antibodies and representative images of hippocampi are shown. (B) Western blot analyses of hippocampal lysates and corresponding quantitated ODs of CD68 (C) and GFAP (D) bands are shown. Values are means ± SDs, n = 4. Means without a common letter differ, P < 0.05. APP/PS1, amyloid precursor protein/presenilin 1; C-15, 3-month study, 15-month-old control mice; CD68, cluster of differentiation 68; GFAP, glial fibrillary acidic protein; P-15, 3-month study, pomegranate-fed 15-month-old mice; 12, untreated 12-month-old control mice.