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. Author manuscript; available in PMC: 2013 Aug 8.
Published in final edited form as: J Biol Chem. 2004 Dec 3;280(9):8079–8085. doi: 10.1074/jbc.M409647200

Figure 5. UV irradiation induces c-Jun and c-Fos in human skin fibroblasts.

Figure 5

Cells were UV-irradiated (50 mJ/cm2) and total RNA, or whole cell protein extracts were prepared at the indicated times post irradiation. A, c-Jun and C, c-Fos mRNA levels were quantified by real-time RT-PCR, normalized to mRNA for 36B4, which encodes a ribosomal protein, used as an internal control. B, c-Jun and D, c-Fos protein levels were determined by Western analysis. Insets show representative Western blots. Bar heights indicate means ± SEM fold change relative to levels in non-irradiated control cells (CTRL). *p<0.05 vs. CTRL. N=3-5.