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. Author manuscript; available in PMC: 2013 Aug 8.
Published in final edited form as: J Biol Chem. 2004 Dec 3;280(9):8079–8085. doi: 10.1074/jbc.M409647200

Figure 6. Over-expression of dominant negative c-Jun abolished UV induction of Smad7 promoter activity in human skin fibroblasts.

Figure 6

Cells were co-transfected with Smad7 promoter/luciferase construct (-613 to +112) and β-galsctosidase expression vector, and empty or dominant negative c-Jun expression vector. Cells were UV irradiated (50mJ/cm2) 24 hours after transfection. Cell lysates were prepared 24 hours post UV irradiation, and promoter activity was determined by luciferase assay, and normalized to β-galactosidase activity. Bars indicate means ± SEM for fold change in Smad7 promoter activity relative to activity in non-irradiated control cells (CTRL). *p<0.05 vs. UV-irradiated cells. N=3.