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. Author manuscript; available in PMC: 2013 Aug 8.
Published in final edited form as: Dev Biol. 2011 Nov 18;361(2):313–325. doi: 10.1016/j.ydbio.2011.10.034

Fig. 4.

Fig. 4

LMO4 forms a complex with Slug and Snail. (A) Co-immunoprecipitation (IP) assay probing the ability of LMO4 to interact with neural crest regulatory factors. Embryos were injected with mRNA encoding flag-tagged LMO4 and indicated myc-tagged neural crest transcription factors. Whole embryo lysates were prepared at stage 10.5, immunoprecipitated with α-flag antibodies, resolved by SDS page and subjected to western analysis using α-myc antibody. LMO4 interacts strongly with Slug and Snail but not Sox10, FoxD3 or Twist. (B) GST pull-down assay demonstrating that in vitro translated LMO4 protein directly interacts with GST-Slug, but not with GST alone.