Fig. 6.

Slug and Snail utilize different domains to recruit Ajuba and HDAC. (A–B) Co-immunoprecipitation (IP) assay comparing the protein domains of (myc-tagged) Slug and Snail required for interaction with flag-tagged HDAC1 (A) or Ajuba (B). Deletion of the SNAG domain of Snail but not Slug, leads to loss of interaction with both HDAC1 and Ajuba. Conversely, the zinc finger domain of Slug, but not Snail, is sufficient for both interactions. These findings reveal novel differences in how Slug and Snail interaction with transcriptional co-regulatory factors.