Skip to main content
. Author manuscript; available in PMC: 2013 Aug 8.
Published in final edited form as: Exp Cell Res. 2007 Dec 23;314(5):1163–1176. doi: 10.1016/j.yexcr.2007.12.012

Figure 1. Effects of resveratrol (RSV) on induction of apoptosis in melanocytes and melanomas.

Figure 1

(A) Cells were stained by PI 16-24 h after treatment. Apoptosis (Ap) levels were determined as the percentage of cells with hypodiploid content of DNA in the pre-G0/G1 region using flow cytometry. % Cells at the distinct phases of the cell cycle is indicated. Results of typical experiments (one of three) are presented. (B) Clonogenic survival assay of LU1205 and LOX cells 12 days after indicated treatment. Error bars represent mean ± S.D. from three independent experiments. (C) Surface expression of TRAIL-R2/DR5 and TRAIL in LU1205 and LOX cells was determined by immunostainig using PE-labeled mAbs and the FACS analysis. (D) RSV induced TRAIL-mediated apoptosis in LOX melanoma cells. Introduction of anti-TRAIL (5 μg/ml), but not anti-FasL inhibitory antibodies suppressed RSV-induced apoptosis in LOX and LU1205 cells, while anti-TNF mAb (5 μg/ml) accelerated apoptosis.