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. Author manuscript; available in PMC: 2013 Aug 8.
Published in final edited form as: Exp Cell Res. 2007 Dec 23;314(5):1163–1176. doi: 10.1016/j.yexcr.2007.12.012

Figure 2. Modulation of RSV-induced apoptosis in LOX human melanoma cells.

Figure 2

(A) Effects of RSV (25-100 μM; 6 h) on NF-κB-, AP-1/cJun- and STAT-dependent luciferase reporter activities, TRAIL and FLIP promoter activities. The reporter constructs used were: 2×NF-κB-Luc, Jun2-Luc, 3×Ly6A/E-Luc (STAT-dependent), 1.5 kb TRAILpr-Luc, 1 kb cFLIPpr-Luc and tk-Luc, as the control vector with tk-promoter. Luciferase reporter activity was normalized based on β-gal activity; β-gal expression construct was cotransfected at a ratio of 1/4. A ratio of the specific luciferase reporter activity to luciferase activity driven by the empty tk-Luc vector in the mock control culture is indicated. Error bars represent mean ± S.D. from three independent experiments. (B) Effects of RSV (50 μM) and Bay11-7082 (5 μM) on basal nuclear NF-κB DNA binding activity in LOX cells determined by EMSA. Positions of DNA-binding complexes and free labeled probe (FP) are indicated. (C, D) Effects of pharmacological inhibitor of NF-κB (Bay 11-7082, 5 μM), on apoptosis and clonogenic survival of LOX cells after RSV treatment. Error bars represent mean ± S.D. from three independent experiments.