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. Author manuscript; available in PMC: 2013 Aug 8.
Published in final edited form as: Exp Cell Res. 2007 Dec 23;314(5):1163–1176. doi: 10.1016/j.yexcr.2007.12.012

Figure 3. Effects of resveratrol (RSV) on cellular proteins controlling cell survival and apoptosis in LU1205 human melanoma cells.

Figure 3

(A, B) Effects of RSV on basal nuclear NF-κB and NF-Y activities in SW1 and LU1205 cells determined by EMSA 6 h after treatment. Positions of DNA-binding complexes are indicated. Free labeled probes are not shown. Western blot analysis was performed for detection of total and phospho-protein levels of STAT3, AKT, FOXO-3A, ERK1/2, JNK1/2, cJun, p38MAPK, ATF2, CREB, TRAIL, TRAIL-R2/DR5, cFLIP, P-(Ser20) p53, total p53, p21-WAF, BAX, Bcl-xL and of Cyclin D1 6 h after treatment with indicated concentrations of RSV. (C) Western blot analysis of Bcl-xL, survivin, XIAP and β-actin levels in LU1205 cells 16 h after treatment with RSV. (D) Effects of RSV (25-100 μM; 6 h) on AP-1/cJun-, NF-κB-, STAT- and p53-dependent luciferase reporter activities, TRAIL and FLIP promoter activities. The reporter constructs used were: Jun2-Luc, 2×NF-κB-Luc, 3×Ly6A/E-Luc (STAT-dependent), p53RE-Luc, 1.5 kb TRAILpr-Luc, 1 kb cFLIPpr-Luc and tk-Luc, as the control vector with tk-promoter. Luciferase reporter activity was normalized based on β-gal activity; β-gal expression construct was cotransfected at a ratio of 1/4. A ratio of the specific luciferase reporter activity to luciferase activity driven by the empty tk-Luc vector in the mock control culture is indicated. Error bars represent mean ± S.D. from three independent experiments. (E) Effects of pharmacological inhibitors of MEK-ERK (U0126, 10 μM), p38 MAPK (SB203580, 10 μM) and JNK (SP600125, 10 μM) on RSV-induced apoptosis in LU1205 cells. Error bars represent mean ± S.D. from three independent experiments.