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. Author manuscript; available in PMC: 2013 Aug 8.
Published in final edited form as: Exp Cell Res. 2007 Dec 23;314(5):1163–1176. doi: 10.1016/j.yexcr.2007.12.012

Figure 6. Upregulation of TRAIL and RSV induced apoptosis in LU1205 melanoma cells.

Figure 6

(A) Combined effects of sodium arsenite (As, 5 μM) and resveratrol (RSV, 50 μM) on DR5 surface expression in LU1205 cells. RSV was added into cell media 1 h after sodium arsenite. Cells were stained 6 h after treatment with PE-labeled anti-human DR5 mAb and analyzed by the flow cytometry; MFI was indicated (B) Combined effects of sodium arsenite (As, 5 μM) and NS398 (50 μM) on DR5 surface expression. (C) Western blot analysis of total protein levels of DR5 and dynamin-2 six h after indicated treatment of LU1205 cells. (D) Acceleration of TRAIL-mediated apoptosis in LU1205 cells after pretreatment with sodium arsenite (5 μM) + NS398 (50 μM) for 16 h, or after γ-irradiation (2.5-5 Gy). TRAIL (50 ng/ml), RSV (50 μM) and CHX (2 μg/ml) were added 16 h after an initial treatment and remained in the media for the next 32 h. Error bars represent mean + S.D. from three independent experiments.