Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Aug 8;9(8):e1003702. doi: 10.1371/journal.pgen.1003702

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 Zhou et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

(A) Schematic showing the inducible dimerization. Addition of AP20187 induces dimerization (interaction) of FKBP-containing proteins. (B) Immunoprecipitation (IP) coupled immunoblot (IB) analysis of HEK293T cells that were transfected with the indicated vectors (Flag-tagged and HA-tagged). Cells were treated with 100 nm of AP20187 for 1 hr and analyzed by IP-IB using indicated antibodies. (C) IP-IB analysis of HEK293T cells after transfection with empty vector (HA-FKBP) or wild type TopBP1 (HA-FKBP-wtTopBP1). Cells at 40 hr after transfection were incubated with 100 nm of AP20187 for 1 hr without or with 1.6 mM of ATR inhibitor (ATRi) or were treated with 100 J/m-2 UV. Immunoblot analysis was performed 1 hr after respective treatment. (D) IP-IB analysis of HEK293T cells after transfection with empty vector (HA-FKBP), or FKBP-fused wild type TopBP1 (HA-FKBP-wtTopBP1), or FKBP-fused AAD mutant TopBP1 (HA-FKBP-mtTopBP1), or wild type TopBP1 without FKBP (HA-wtTopBP1). Cells were treated with 100 nm of AP20187 for 1 hour then analysed by immunoblotting with indicated antibodies.