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. 2013 Aug 8;8(8):e71228. doi: 10.1371/journal.pone.0071228

Figure 2. Soluble recombinant DO recognizes soluble recombinant DM.

Figure 2

(A) DO (Ni-NTA purified) was immobilized on an anti-His antibody surface (blue trace). After a brief wash, DM was injected over the captured DO (green trace). A control injection of DM over anti-His antibody surface (red trace) showed no non-specific binding. Data shown are representative of six independent experiments. (B) DO (Ni-NTA purified) binding to DM immobilized by anti-FLAG antibody surface in concentrations ranging from 0.01 to 10 µM in separate experiments.