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. 2013 Aug 8;8(8):e71228. doi: 10.1371/journal.pone.0071228

Figure 6. HLA-DO affects peptide binding to DR1 independent of DM.

Figure 6

(A) Association kinetics of HA(306–318) to peptide-receptive DR1 without DO (black triangles) or with DO (red triangles). (B) Association kinetics of CII(259–273) to peptide-receptive DR1 without DO (black triangles) or with DO (red triangles). Experiments in A and B are representative of three-four trials. (C) HA(anchorless) association to constitutively receptive mutant DR1βG86Y molecules with no accessory molecules (black squares), with DM (red dots), DO (green triangles) or both DO and DM (blue triangles). The fluorescence signals (Arbitrary Fluorescence Units) associated with the control samples incubated >10 hours in the absence of DR1 were measured: HA(anchorless) peptide alone, 1804; HA(anchorless)+DM, 1512; HA(anchorless)+DO, 2280; HA(anchorless)+DM+DO, 2888.