Table 1. The initial rates of peptide/DR1 complex formation for tested peptides with and without accessory molecules.
Observed Initial Binding Rates in interaction of DR1 with Fluorescent Peptides# | ||||
Peptides | DR1 alone | + DM | + DO | + DM and DO |
HA(306-318) | 132 | 463 | 523 | 1779 |
HA(Y308A)* | 118 | 331 | 184 | 234 |
HA(anchorless) | 803 | 2141 | 385 | 651 |
H5N1-HA1(259-274) | 98 | 665 | 257¶ | 2197 |
CII(281-295) | 500 | 1593 | 137 | 1002 |
Initial Rates were determined for 1 hour (Response; Arbitrary Fluorescence Units min−1).
The effect of DO on HA(Y308A) was determined by using co-expressed DM/DO complex.
Determined in a separate experiment.