Table 2. Bacterial strains and plasmids used in this study.
Strain or plasmid | Characteristic(s) a | Source or reference |
---|---|---|
MG1363 | L. lactis subsp. cremoris ; Lac- PrtP-; plasmid-free derivative of NCDO712 | [13] |
MG1363acmAΔ1 | Derivative of MG1363 carrying a 701-bp SacI-SpeI deletion in acmA | [7] |
NZ9000 | MG1363pepN::nisRK | [23] |
NZ9000acmAΔ1 | Derivative of NZ9000 with a 701-bp SacI-SpeI deletion in acmA | [43] |
NZ9700 | Nisin-producer | [23] |
LL108 | MG1363 carrying multiple copies of the pWV01 repA gene in the chromosome | [27] |
IL1403 | L. lactis subsp. lactis ; plasmid-free | [10] |
IL1403acmA::ISS1 | Insertion of transposon ISSI in acmA | [40] |
IL1403acmA::ISS1acmD::myc | Derivative of IL1403acmA::ISSI with an in-frame integrated c-myc epitope in acmD | This work |
EC101 | Kmr; E. coli JM101 with repA from pWV01 integrated in the chromosome | [24] |
MC1061 | E. coli araD139 Δ(araA-leu)7697 ΔlacX74 galK16 galE15(GalS) λ- e14- mcrA0 relA1 rpsL150(strR) spoT1 mcrB1 hsdR2 | [14] |
pVE6007 | Cmr, Ts derivative of pWV01 | [31] |
pORIacmD::myc | Emr, LacZ, pORI280acmD with c-myc epitope inserted into the BamHI site | This work |
pNG304 | Cmr, pNZ8048 derivative containing the pre-pro sequence of prtP fused to msa2 under control of PnisA | [41] |
pNG3041 | Cmr, pNZ8048 derivative containing the pre-pro sequence of prtP fused to msa2 and the C-terminal domain of acmA under control of PnisA | [41] |
pNG3042 | Cmr, pNZ8048 derivative containing the pre-pro encoding sequence of prtP fused to msa2 and the C-terminal domain encoding sequence of acmD under control of PnisA | [5] |
pBAD | Ampr, carrying arabinose-inducible promoter ParaBAD | [15] |
pBADcLIC | Ampr, pBAD derivative carrying arabinose promoter and His10 tag | [14] |
pBADcLIC-GFP | Ampr, pBAD derivative carrying arabinose promoter and gfp fused to the His10 tag encoding sequence | [14] |
pBADcLIC-GFP-LysMAcmD | Ampr, pBADcLIC-GFP with sequence encoding the 3 LysM repeats of AcmD in SwaI site | This work |
pBADcLIC-GFP-LysMAcmA | Ampr, pBADcLIC-GFP with sequence encoding the 3 LysM repeats of AcmA in SwaI site | [49] |
PrtP- = proteolytically inactive
Ts = thermosensitive
Ampr, Cmr, Emr = resistance to ampicillin, chloramphenicol and erythromycin, respectively