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. 2013 Aug 8;8(8):e72167. doi: 10.1371/journal.pone.0072167

Table 2. Bacterial strains and plasmids used in this study.

Strain or plasmid Characteristic(s) a Source or reference
MG1363 L. lactis subsp. cremoris ; Lac- PrtP-; plasmid-free derivative of NCDO712 [13]
MG1363acmAΔ1 Derivative of MG1363 carrying a 701-bp SacI-SpeI deletion in acmA [7]
NZ9000 MG1363pepN::nisRK [23]
NZ9000acmAΔ1 Derivative of NZ9000 with a 701-bp SacI-SpeI deletion in acmA [43]
NZ9700 Nisin-producer [23]
LL108 MG1363 carrying multiple copies of the pWV01 repA gene in the chromosome [27]
IL1403 L. lactis subsp. lactis ; plasmid-free [10]
IL1403acmA::ISS1 Insertion of transposon ISSI in acmA [40]
IL1403acmA::ISS1acmD::myc Derivative of IL1403acmA::ISSI with an in-frame integrated c-myc epitope in acmD This work
EC101 Kmr; E. coli JM101 with repA from pWV01 integrated in the chromosome [24]
MC1061 E. coli araD139 Δ(araA-leu)7697 ΔlacX74 galK16 galE15(GalS) λ- e14- mcrA0 relA1 rpsL150(strR) spoT1 mcrB1 hsdR2 [14]
pVE6007 Cmr, Ts derivative of pWV01 [31]
pORIacmD::myc Emr, LacZ, pORI280acmD with c-myc epitope inserted into the BamHI site This work
pNG304 Cmr, pNZ8048 derivative containing the pre-pro sequence of prtP fused to msa2 under control of PnisA [41]
pNG3041 Cmr, pNZ8048 derivative containing the pre-pro sequence of prtP fused to msa2 and the C-terminal domain of acmA under control of PnisA [41]
pNG3042 Cmr, pNZ8048 derivative containing the pre-pro encoding sequence of prtP fused to msa2 and the C-terminal domain encoding sequence of acmD under control of PnisA [5]
pBAD Ampr, carrying arabinose-inducible promoter ParaBAD [15]
pBADcLIC Ampr, pBAD derivative carrying arabinose promoter and His10 tag [14]
pBADcLIC-GFP Ampr, pBAD derivative carrying arabinose promoter and gfp fused to the His10 tag encoding sequence [14]
pBADcLIC-GFP-LysMAcmD Ampr, pBADcLIC-GFP with sequence encoding the 3 LysM repeats of AcmD in SwaI site This work
pBADcLIC-GFP-LysMAcmA Ampr, pBADcLIC-GFP with sequence encoding the 3 LysM repeats of AcmA in SwaI site [49]
a

PrtP- = proteolytically inactive

Ts = thermosensitive

Ampr, Cmr, Emr = resistance to ampicillin, chloramphenicol and erythromycin, respectively