Table 1. List of potential off-targets for pico inverted repeat constructs.
| picoRNAiIR4 | picoRNAiR2 and picoRNAiR3 | ||||||||
| mis = 0 | mis = 1 | mis = 2 | FlyBase ID | CG number | mis = 0 | mis = 1 | mis = 2 | FlyBase ID | CG number |
| 459 | 0 | 0 | FBgn0261811 | CG11940-PB | 482 | 4 | 61 | FBgn0261811 | CG11940-PA |
| 459 | 0 | 0 | FBgn0261811 | CG11940-PA | 339 | 5 | 48 | FBgn0261811 | CG11940-PB |
| 0 | 2 | 7 | FBgn0022787 | CG4261-PA | 5 | 6 | 30 | FBgn0052082 | CG32082-PA |
| 0 | 2 | 3 | FBgn0031988 | CG8668-PA | 5 | 6 | 20 | FBgn0045852 | CG31753-PA |
| 0 | 2 | 2 | FBgn0027866 | CG9776-PA | 5 | 4 | 28 | FBgn0261885 | CG7467-PB |
| 0 | 2 | 2 | FBgn0021760 | CG32435-PA | 5 | 4 | 28 | FBgn0261885 | CG7467-PA |
| 0 | 2 | 2 | FBgn0033460 | CG1472-PA | 5 | 4 | 27 | FBgn0261885 | CG7467-PC |
| 0 | 2 | 2 | FBgn0021760 | CG32435-PB | 5 | 2 | 16 | FBgn0085405 | CG31160-PA |
| 0 | 2 | 2 | FBgn0021760 | CG32435-PC | 5 | 2 | 2 | FBgn0039260 | CG6369-PA |
| 0 | 2 | 2 | FBgn0027866 | CG9776-PB | 4 | 5 | 7 | FBgn0264895 | CG6682-PA |
| 0 | 1 | 7 | FBgn0052251 | CG32251-PA | 4 | 2 | 7 | FBgn0004889 | CG6235-PC |
| 0 | 1 | 5 | FBgn0031116 | CG1695-PB | 4 | 2 | 7 | FBgn0004889 | CG6235-PE |
| 0 | 1 | 5 | FBgn0031116 | CG1695-PA | 4 | 2 | 7 | FBgn0004889 | CG6235-PD |
| 0 | 1 | 5 | FBgn0263289 | CG5462-PA | 4 | 2 | 7 | FBgn0004889 | CG6235-PH |
| 0 | 1 | 5 | FBgn0263289 | CG5462-PD | 4 | 2 | 7 | FBgn0004889 | CG6235-PB |
| 0 | 1 | 5 | FBgn0263289 | CG5462-PB | 4 | 2 | 7 | FBgn0004889 | CG6235-PG |
| 0 | 1 | 5 | FBgn0263289 | CG5462-PC | 3 | 20 | 64 | FBgn0037252 | CG14650-PA |
| 0 | 1 | 3 | FBgn0033558 | CG12344-PA | 3 | 6 | 32 | FBgn0026718 | CG17608-PA |
| 0 | 1 | 3 | FBgn0039554 | CG5003-PA | 3 | 3 | 10 | FBgn0038412 | CG6898-PA |
| 0 | 1 | 3 | FBgn0261388 | CG15720-PA | 3 | 2 | 11 | FBgn0052333 | CG32333-PA |
| 0 | 1 | 3 | FBgn0053519 | CG30175-PA | 3 | 2 | 5 | FBgn0263144 | CG8276-PA |
| 0 | 1 | 3 | FBgn0031299 | CG4629-PC | 3 | 2 | 5 | FBgn0263144 | CG8276-PB |
| 0 | 1 | 3 | FBgn0028474 | CG4119-PA | 2 | 21 | 45 | FBgn0034072 | CG18250-PC |
| 0 | 1 | 3 | FBgn0024329 | CG7717-PA | 2 | 7 | 14 | FBgn0037503 | CG14598-PA |
| 0 | 1 | 3 | FBgn0024329 | CG7717-PB | 2 | 4 | 13 | FBgn0261938 | CG4644-PA |
| 0 | 1 | 3 | FBgn0031299 | CG4629-PB | 2 | 4 | 3 | FBgn0028380 | CG9670-PA |
| 0 | 1 | 3 | FBgn0031299 | CG4629-PA | 2 | 3 | 14 | FBgn0004435 | CG17759-PA |
| 0 | 1 | 2 | FBgn0051301 | CG31301-PA | 2 | 3 | 14 | FBgn0004435 | CG17759-PG |
| 0 | 1 | 2 | FBgn0028647 | CG11902-PA | 2 | 3 | 8 | FBgn0263396 | CG16901 |
| 0 | 1 | 2 | FBgn0037391 | CG2017-PB | 1 | 22 | 117 | FBgn0016694 | CG17888-PD |
Off-targets were determined using dscheck (http://dscheck.rnai.jp/), which generates all possible 19 mers that can be theoretically generated from a longer dsRNA sequence and determines their homology to Drosophila genes identified by their Flybase ID and Celera Genomics (CG) number. The number of 19 mers matching each gene found by the sequence comparison are tabulated, where Mis = 0, Mis = 1, Mis = 2 correspond to the number of 19 mers with 0, 1 and 2 mismatches for each gene, respectively.