Table 1. Primers designed for the different clonings of the wcaI/J and wzc/wcaA fragments in pBAD33 and pBAD18 (see Fig. 2).
Name | Sequence | Restriction |
wcaI_F02 | GGGGTACCCCggagtgAAACGATG AAAATACTGGTCTACGGC | KpnI |
wcaI_R01 | AACTGCAGTTTATCCCCGAATATCATTTATAAATTG | PstI |
cpsB_F02 | GGGGTACCCCggagtgAAACGATG GCGCAGTCGAAACTCTATCC | KpnI |
cpsB_R01 | AACTGCAGTTTACACCCGTCCGTAGCGATCC | PstI |
cpsG_F02 | GGGGTACCCCggagtgAAACGATG AAAAAATTAACCTGCTTTAAAGC | KpnI |
cpsG_R01 | AACTGCAGTTTA CTCGTTCAGCAACGTCAGCAG | PstI |
wcaJ_F02 | GGGGTACCCCggagtgAAACGATG ACAAATCTAAAAAAGCGCGAGC | KpnI |
wcaJ_R01 | AACTGCAGTTCA ATATGCCGCTTTGTTAACGAAACC | PstI |
wzc_F01 | GGGGTACCCCggagtgAAACGATGACAGAAAAAGTAAAACAACATGCC | KpnI |
wzc_R01 | GCTCTAGA TTATTTCGCATCCGACTTATATTCG | XbaI |
wcaA_F01 | GGGGTACCCCggagtgAAACGATGAAAAACAATCCGCTGATCTCAATC | KpnI |
wcaA_R01 | GCTCTAGA TTAGCGCCCCCGAATACCATCAG | XbaI |
On the sequence of the primers is represented: the restriction site (underlined); the Shine Dalgarno (lower case letter); start or stop codon (bold) for the forward (F symbol in the primer name) or reverse (R symbol) primers, respectively.