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. 2013 Aug 8;8(8):e71097. doi: 10.1371/journal.pone.0071097

Table 1. Primers designed for the different clonings of the wcaI/J and wzc/wcaA fragments in pBAD33 and pBAD18 (see Fig. 2).

Name Sequence Restriction
wcaI_F02 GGGGTACCCCggagtgAAACGATG AAAATACTGGTCTACGGC KpnI
wcaI_R01 AACTGCAGTTTATCCCCGAATATCATTTATAAATTG PstI
cpsB_F02 GGGGTACCCCggagtgAAACGATG GCGCAGTCGAAACTCTATCC KpnI
cpsB_R01 AACTGCAGTTTACACCCGTCCGTAGCGATCC PstI
cpsG_F02 GGGGTACCCCggagtgAAACGATG AAAAAATTAACCTGCTTTAAAGC KpnI
cpsG_R01 AACTGCAGTTTA CTCGTTCAGCAACGTCAGCAG PstI
wcaJ_F02 GGGGTACCCCggagtgAAACGATG ACAAATCTAAAAAAGCGCGAGC KpnI
wcaJ_R01 AACTGCAGTTCA ATATGCCGCTTTGTTAACGAAACC PstI
wzc_F01 GGGGTACCCCggagtgAAACGATGACAGAAAAAGTAAAACAACATGCC KpnI
wzc_R01 GCTCTAGA TTATTTCGCATCCGACTTATATTCG XbaI
wcaA_F01 GGGGTACCCCggagtgAAACGATGAAAAACAATCCGCTGATCTCAATC KpnI
wcaA_R01 GCTCTAGA TTAGCGCCCCCGAATACCATCAG XbaI

On the sequence of the primers is represented: the restriction site (underlined); the Shine Dalgarno (lower case letter); start or stop codon (bold) for the forward (F symbol in the primer name) or reverse (R symbol) primers, respectively.