Figure 7. Averaged trace of the Ca²⁺-mobilization by thapsigargin in H295R.

Each point with an error bar represents the Mean±SD (N = 6). Thapsigargin (TG), a calcium ATPase inhibitor of endoplasmic reticulum, was applied during the period indicated by solid bars. White and lined columns indicate free of extracellular Ca²⁺ and 1.2 mM Ca²⁺ conditions respectively. TG (2 µM) significantly increased the Ca²⁺-free basal ratio (0.14+0.007) to 0.18+0.011 (p<0.001) transiently. The 2nd Ca²⁺ peak was represented by the addition of 1.2 mM CaCl₂ to the assay media.