Figure 3. Chronic blockade of CaM-Kinase IIa mimics the effect of NMDA-receptor blockade on EPSCs in neurons expressing NL1.
Sample traces (A) and summary graphs (B) of EPSCs recorded from neurons expressing only EGFP or EGFP-tagged NL1 cultured either in medium containing 5 μM KN-93 ( CaM-Kinase IIα inhibitor) or in control medium containing 5 μM KN-92 ( inactive analog of KN-93) for four days prior to the recordings. Data shown are means ± SEMs; asterisks represent statistically significant differences (n=18 cells/3 cultures; *=p<0.05, ** =p<0.01; ns, not significantly different).