During immune synapsis, the molecules involved in antigen recognition (TCR and peptide-loaded-MHC class II) locate at a central cluster surrounded by a peripheral ring enriched in adhesion molecules (integrin LFA-1 and ICAMs) and the actin cytoskeleton. The T lymphocyte orients its MTOC and secretory compartments (Golgi apparatus and MVBs) toward the APC. We propose that the IS provides a more efficient path for the exchange of genetic material through the combination of different mechanisms, including the polarized secretion of microRNA-loaded exosomes, trans-endocytosis and membrane bridges. Pathogens, including bacteria and viruses, hijack biological synapses to spread from cell to cell. APC, antigen presenting cell; MVB, multivesicular body.